BCL2 is a tumor suppressor gene located in the 18q21 region. BCL2 gene encodes a mitochondrial membrane protein that regulates apoptosis and is expressed in B cells. Translocation of the BCL2 gene is usually recognized in B cell lymphoma. In particular, translocation of t(14;18)(q32.3;q21.3) is present in approximately 80% of follicular lymphoma (FM), 20%-30% of diffuse large B-cell lymphoma In (DLBCL), it rarely occurs in B-cell chronic lymphocytic leukemia (B-CLL). Therefore, the detection of BCL2 translocation by fluorescence in situ hybridization (FISH) may have diagnostic and prognostic significance.
BCL2 is a dual-color break apart probe composed of two probes directly labeled at 18q21.33-q22.1. The green fluorescent dye labeled probe hybridizes to the proximal end of the BCL2 gene, while the orange-red fluorescent dye labeled probe hybridizes to the distal end of the BCL2 gene.
Follicular lymphoma (FL) is a less malignant B cell tumor derived from the center of follicle development. FL is a common type of non-Hodgkin’s lymphoma (NHL), accounting for about 10% of NHL in China and 25%-45% of NHL in Europe and America. BCL2 gene break apart and MTC gene break apart can be used both in the lymphoma diagnosis.
Product size: 100μL FISH probe ( ⬤ | ⬤ ) + Pretreatment reagent (10 tests) + Antifade staining solution DAPI (10 tests).